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International Journal of Research and Analytical Reviews (IJRAR)

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E-ISSN 2348-1269, P- ISSN 2349-5138 | ESTD Year: 2014

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  Paper Title  

DETERMINATION OF PHYTOCOMPOUNDS FROM METHANOL EXTRACTS OF GANODERMA LUCIDUM BY LC-MS

  Authors

  Vinotha. V,  Rajakumar. R

  Keywords

Phytochemical, Ganoderma lucidum, FT-IR, Methanol extracts and Ionization.

  Abstract


Ganoderma is an herb that has been in use for more than 2000 years to alleviate a great numbers of health complaints. Thus the present study focused on the qualitative determination of phytochemicals, extraction and purification of bioactive compounds from Ganoderma lucidum. The preliminary phytochemical screening showed positive reactions for alkaloid, flavanoid, terpenoid, steroid, reducing sugars and carbohydrates tests except saponins. The LC-MS/MS study reported that totally 13 compounds were screened based on negative ionization [M-H]- and also 12 peaks were identified from FTIR spectrum with their functional groups. The peak showed (M-H)- ion at m/z 169 indicate the Gallic acid. The compound Theanine, Caffeic acid and Caffeine were assigned for peak as the MS pattern depicted a parent ion at m/z 174.2, 183.1 and 194.1respectively. The FT-IR spectrum was used to identify the functional groups of the active components present in extract based on the peaks values in the region of IR radiation. IR spectrum of the isolated compound was registered a broad band cm-1 and stretching band at 3277.49 to 410 cm-1. Some bands in the FTIR spectrum of methanol extracts of Ganoderma lucidum showed different bands at range of 3277.49, 2923.05, 1636.19, 1373.48, 1033.1, 656.57, 589.71, 553.03, 520.68, 485.73, 433.75 and 410 cm-1. A strong peak at 3277.49 cm-1 in Ganoderma lucidum corresponds to O-H/C-H stretching of the carboxylic acid group.

  Cite this article

  Vinotha. V,  Rajakumar. R,   "DETERMINATION OF PHYTOCOMPOUNDS FROM METHANOL EXTRACTS OF GANODERMA LUCIDUM BY LC-MS", IJRAR - International Journal of Research and Analytical Reviews (IJRAR), E-ISSN 2348-1269, P- ISSN 2349-5138, Volume.6, Issue 1, Page No pp.914-918, March 2019, Available at : http://www.ijrar.org/IJRAR19H1136.pdf  

  IJRAR's Publication Details

  Unique Identification Number - IJRAR19H1136

  Paper ID - 200981

  Page Number(s) - 914-918

  Pubished in - Volume 6 | Issue 1 | March 2019

  DOI (Digital Object Identifier) -   

  Publisher Name - IJRAR | www.ijrar.org | E-ISSN 2348-1269, P- ISSN 2349-5138

  E-ISSN 2348-1269, P- ISSN 2349-5138

  Cite this article

  Vinotha. V,  Rajakumar. R,   "DETERMINATION OF PHYTOCOMPOUNDS FROM METHANOL EXTRACTS OF GANODERMA LUCIDUM BY LC-MS", IJRAR - International Journal of Research and Analytical Reviews (IJRAR), E-ISSN 2348-1269, P- ISSN 2349-5138, Volume.6, Issue 1, Page No pp.914-918, March 2019, Available at : http://www.ijrar.org/IJRAR19H1136.pdf

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The IJRAR - International Journal of Research and Analytical Reviews (IJRAR) aims to explore advances in research pertaining to applied, theoretical and experimental Technological studies. The goal is to promote scientific information interchange between researchers, developers, engineers, students, and practitioners working in and around the world.


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